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KMID : 0378019630060110091
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New Medical Journal
1963 Volume.6 No. 11 p.91 ~ p.108
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Effect of Radioactive Phosphorus, P^32, on the Pregnant Albino Rat and its Fetus
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Abstract
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In order to investigate the effect of radioactive phosphorus, Ps´ on the pregnant albino rat and its fetus, experimentation was carried out by irtraperitoneal injection of P32 in doses of 0.5. 1.0. 1.5 and 2.0 mc, respectively, to rats, 7 and 14 days pregnant, using non-pregr ant rats as a control. After this treatment, measurement of body weight, cyclic change of vaginal smear, behavior of pregnancy course, and histopathological alteration of major organs in the mother rat, such as heart, lung, liver,. spleen, kidney, adrenals, ovaries and uterus were analyzed.
The radioactive phosphorus resulted in fetal death and/or complete disappearance of fetal tissue in some instances and in interim developmental changes in others.
The results of this investigation are as follows:
1. An examination of cyclic change on vaginal smear is of considerable value in that in most instances an early confirmation of pregnancy in rats is established. Stage-V, the non-estrus stage, persists throughout the entire course of pregnancy.
2. In all groups of P32 treated pregnant rats, reduction in body weight was observed. Weight loss of pregnant rats was more pronounced in the group treated with P32 in the early part of pregnancy (7-days pregnant group) than in the group treated with P32 in the later part of pregnancy (14-days pregnant group).
3. In non-pregnant rats the cyclic change of vaginal smear was shown to be considerably inhibited by P -´ treatment. In the group of non-pregnant rats treated with a dose above 1.5 me of P32, the non-estrus stage, stage-V, lasted for several days after P37 injection, whereas a similar nor,-pregnant rats group treated with less than 1.0 mc. of P32 demonstrated irregularity of vaginal cyclic change with the tendency to observe occasional persistent stage-1 and/or stage-V.
In pregnant rats, stage-V persisted throughout the entire course of pregnancy and therefore it was not possible to evaluate the cyclic change at all.
4. Maternal death occurred (5 out of 6 rats) only in the group treated with 1.5 inc. bf P32.on"thi-46 day of pregnancy. No death resulted in the group of rats treated with 1.5 mc. of P32 on the 14th day´´ of pregnancy or in non-pregnant groups.
5. Radioactive destructive effect of P32 to the fetal rats (fetal death and/or complete disappearance of fetal tissue) varied with respect to dosage of P32 administered and duration of pregnancy, the des. tructive effect being more pronounced in the group of rats with 7-days pregnancies than with those with 14-days pregnancies.
6. So far as histopathological findings are concerned, no marked differences were observed between pr. egnant and non-pregnant groups treated with P32.
7. Organic change resulting from radioactive phosphorus in the experimental animals reached its peak on the 5th to 7th day after intraperitoneal administration of P32. No evidence of regeneration was obtainable by the 14th day after radioactive phosphorus administration, at which time the animal was sacrificed.
8. The magnitude of organic change as a result of radioactive P32 administration in the experimental animals differed greatly according to the doses of p32 administered, the change being more marked in the group treated with 1.5 mc. of P32 than in the group treated with less than 1.0 mc. of P32. Necrotic changes were apparent in the former group in spleen, bone marrow, lymph node, thymus, and ovaries along with focal necrosis of liver as well as atrophic changes of adrenal glands in the glomerular zone. In the latter group, however, the necrotic changes in organs described above were only slight, and no focal necrosis was present in the liver, nor was there atrophic change in the glomerular zone of adrenal glands.
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KEYWORD
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